Confocals
THOR - Nikon A1 laser scanning, structured illumination and TIRF
- Lasers & Filters
- Objective lenses
- Sensors
- Features & Capabilities
- Software
- Pics
- User Guide
- Reserve Me
Lasers:
Lauch: LUNV6, high-output.
Excitation at: 405, 445, 488, 515, 561, 647 (nm)
Emission (Chroma) and molecules imaged:
- Emission filter: 425-475 (nm): DAPI, Hoechst, DyLight-405, Alexa405, etc.
- Emission filter: 467.5-502.5 (nm): CFP, cerulean, etc.听
- Emission filter: 500-550 (nm): Alexa488, FITC, EGFP, Opal520, etc.听
- Emission filter: 521.5-554.5 (nm): YFP, Venus, etc.听
- Emission filter: 575-625 (nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc.听
- Emission filter: 650-720 (nm): Alexa647, Cy5, Opal620, etc.听
- DIC imaging possible (laser scanning confocal only)
Filters for widefield:
DAPI, GFP, RFP, Brightfield
N-SIM certified objectives:
100x 1.49NA OIL SR Apo TIRF WD 0.12 (mm)
60X 1.27NA Water SR Plan Apo WD 0.17 (mm)
Standard objectives:
- 4x 0.2NA Plan Apo听位 WD 20 (mm)
- 10x 0.45NA Plan Apo 位 WD 4 (mm)
- 20x 0.75NA Plan Apo VC 位 WD 1 (mm)
- 40x 0.6NA S Plan Fluor ELWD 3.6-2.8 (mm)
- 40x 0.95NA Plan Apo 位 WD 0.25-0.17 (mm)
Available upon request:听
- 60x 1.40NA Plan Apo OIL VC 位 WD 0.13 (mm)
Laser Scanning Confocal:
- 2 x GaAsP high-sensitivty PMTs (CFP, GFP, YFP, RFP)
- 2 x multi-alkali PMTs (DAPI, far-red)
N-SIM:
听512 X 512 16 bit
听
听
- TOKAI-HIT Cell Incubator 1 x 35 (mm) dish or chamber side (coverslip bottom)
- Motorized X, Y, Z and separate Mad City Labs Piezo Z
- Multi-point, time-lapse, optical sectioning, large-area montage with stitching
- Increase power over imaging depth
- 听5.21.03; 64 bit
BLACK WIDOW - Nikon Ti Eclipse spinning disk with FRAP
- Lasers & Filters
- Objective lenses
- Sensors
- Features & Capabilities
- Software
- Pics
- User Guide
- Reserve Me
Lasers
- 405, 488, 561, 640听(nm)
- Quad used for excitation transmission in photobleaching or photoactivation experiments听is Semrock Brightline Di01-T405/488/568/647
Emission (Single bandwidth are Chroma and molecules imaged:
- Emission filter: 430-480听(nm): DAPI, Hoechst, DyLight-405, Alexa405, BFP, etc.
- Emission filter: 500-540 (nm): Alexa488, FITC, EGFP, Opal520, etc.听
- Emission filter: 602-662听(nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc.听
- Emission filter: 669-741听(nm): Alexa647, Cy5, Opal620, etc.听
Standard objectives:
- 10x 0.45NA Plan Apo 位 WD 4 (mm)
- 20x 0.75NA Plan Apo VC 位 WD 1 (mm)
- 40x 0.95NA Plan Apo 位 WD 0.25-0.17 (mm)
- 60x 1.40NA Plan Apo VC 位 OIL WD 0.13 (mm)
- 100x 1.45NA Plan Apo 位 OIL WD 0.13 (mm)
Available upon request:听
- 4x听0.13NA Plan Fluor WD 17.2 (mm)
- 60x听1.27NA water SR Plan Apo WD 0.17 (mm)
- Hamamatsu ImagEM EM-CCD (widefield)
- Both cameras are equipped with 512 X 512 16-bit imaging array
- Fast Acquisition
- Multipoint, montaging, stiching
- Single point FRAP/PA with 405 nm听laser (50mW)
- Motorized X, Y, Z
- TOKAI-HIT Cell Incubator; 1 X 35 (mm) dish or chamber slide (coverslip bottom).
听
Chronos - Yokogawa & Olympus CellVoyager鈩 CV1000 spinning disk scanner
Lasers
- 405, 488, 561 (nm) & Brightfield via far-red LED
Emission filters are from Olympus and molecules imaged:
- Emission filter: 447-507 (nm): DAPI, Hoechst, BFP, etc..
- CFP has been imaged using the 405 nm laser with 447-507 (nm) emission filter.
- Emission filter: 500-550听(nm): Alexa488, FITC, EGFP, etc.
- Venus has been imaged using the 488 nm laser with 500-550 (nm) emission filter.
- Emission filter: 580-654听(nm): Alexa568, 594, TxRed, TRITC, mRFP, mCherry, mKO, TdTomato. etc.听
Standard objectives:
- 10x 0.4NA UPLSAPO WD 3.1 (mm)
- 20x 0.75NA UPLSAPO WD 0.65 (mm)
- 20x听0.45NA听LUCPLFLN LWD WD 6.4-7.6 (mm)
- 40x 0.6NA LUCPLFLN LWD WD 2.7-4.0 (mm)
- 40x 1.30NA UPLFLN OIL WD 0.2 (mm)
- 100x 1.40NA UPLSAPO OIL WD 0.13 (mm)
Objectives available upon request:
- 4x 0.13NA UPlanFl N UIS2 WD 17 (mm)
- 60x 1.20NA UPLSAPO WATER WD 0.28 (mm)
- 60x 1.35NA UPLSAPO OIL WD 0.15 (mm)
- This is a 1024 x 1024, 16 bit detector with 13 x 13听micron pixels
- Back thinned FT CCD with >90% QE from 500-700 (nm)
- Cooled to -55 degrees C to reduce detector dark noise
- Camera binning 2x2, 4x4 to increase sensitivity
The nitty gritty
Chronos听is equipped with an ultra-sensitive, high-resolution听mode Hamamatsu EMCCD camera听and a Yokogawa X1 spinning disk with the option of听dual microlens-enhanced Nipkow scanning disks with pinholes of听50 渭m or 25 渭m; disks can also be removed for widefield imaging. Zero Drift (Olympus) autofocusing and software focusing.听This microscope has minimal听phototoxicity and photobleaching, and听is therefore great for live cell imaging at high temporal resolution, multi-point, and multi-day time-lapse. Multi-point montaging of large tissue samples in X-Y-Z and low-to-medium throughput screening. Excellent brightfield imaging through the disk. Can reload previous sample map and imaging positions to re-image the same locations at different magnifications or over time.
Standard sample holders
- Standard slides
- Multiwell slides
- Multiwell plates, including 6, 24, 96 and 384 wells听(please inquire, there are some plate restrictions for live-imaging)
Environmentally controlled chambers
- Single 35 (mm) dish
- Triple 35 (mm) dish
- Multiwell plates including 6, 24, 96 and 384 wells. Please note: this incubation chamber can only accept low profile plates, standard profile do not fit into the chamber.听Please inquire.
Acquisition
- CV1000 software 1.06.06
Viewer
- CV1000 viewer software version 1.06.06 available for users (please email Director or Specialist for copy)
- ImageJ
听
IRON MAN - Zeiss 510 laser scanning confocal - OUT OF SERVICE
- Lasers & Filters
- Objective lenses
- Sensors
- Features & Capabilities
- Software
- Pics
- User Guide
- Reserve Me
Lasers
- Argon/2 - 458, 477, 488, 514 (nm)
- HeNe - 543, 633 (nm)
- Diode - 405/30 (nm)
Molecules imaged:
- DAPI, Hoechst, similar
- CFP
- Alexa488, FITC, EGFP
- Alexa568, 594, TxRed, TRITC, mRFP, mKO
- Alexa647, Cy5
Filters
- DAPI, EGFP, RFP, Brightfield
Standard objectives
- 5x 0.15NA EC Plan-Neofluar WD 3.1 (mm)
- 10x 0.30NA EC Plan-Neofluar WD 4.0 (mm)
- 20X 0.80NA Plan Apochromat WD 0.5 (mm)
- 40x 1.30NA EC Plan-Neofluar OIL WD 0.21 (mm)
- 63x 1.40NA Plan Apochromat OIL WD 0.19 (mm)
- 100x 1.40NA Plan Apochromat OIL WD 0.17 (mm)
- 3x PMT, standard
- Multichannel听
- FRET
- FRAP
- Z-Drive
- 听2009